Mitochondrial Dysfunction in Skin and Ocular Surface Disease: An Interdisciplinary Review

Omeed M Memar1*, Benjamin Caughlin2 and Ali Djalilian3 1Department of Dermatology and Skin Cancer Institute, USA 2Department of Surgery and Division of Otolaryngology, USA 3Department of Ophthalmology, University of Illinois, USA *Corresponding author: Omeed M Memar, Department of Dermatology and Skin Cancer Institute, Chicago, Illinois, USA. To Cite This Article: Omeed M Memar*, Benjamin Caughlin, Ali Djalilian. Mitochondrial Dysfunction in Skin and Ocular Surface Disease: An Interdisciplinary Review. Am J Biomed Sci & Res. 2019 1(4). AJBSR.MS.ID.000538. DOI: 10.34297/AJBSR.2019.01.000538 Received: February 20, 2019 | Published: February 27, 2019 Copy Right@ Omeed M Memar Review Article American Journal of Biomedical Science & Research www.biomedgrid.com


Introduction
Mitochondrial disease is known to affect many aspects of ocular health: the retina, neural elements, carcinogenesis, glaucoma and the ocular surface [1]. The ocular surface comprises of the cornea and conjunctiva and is heavily influenced by the glandular elements present. Here we will focus on the role of mitochondria in ocular surface pathology.
Mitochondria are sub-cellular organelles with dsDNA. Mitochondrial DNA (mt DNA) is normally present at thousands of copies per cell and is packaged into several hundred higher-order structures termed nucleoids MtDNA makes only 13 proteins, a noncoding regulatory D-Loop, 2rRNAs and 22 tRNAs, including genes for 13 oxidative phosphorylation (OXPHOS) polypeptides, 22 tRNAs and two ribosomal RNAs, while some of the OXPHOS elements are encoded by nuclear DNA [2]. The function of mitochondria is energy (ATP) formation, heme synthesis, calcium homeostasis, cell signaling, cellular differentiation, cell growth, cell cycling, and cellular death [3]. Here we go deeper into the function and dysfunction of the mitochondria and the ramifications it has on the ocular surface ( Figure 1).

Function of mitochondria
Through OXPHOS, mitochondria use breakdowns of proteins, fatty acids and sugars to generate using c5 ( Figure 1). Mitochondria are thought to be of bacterial origin and mitochondrial function is driven by mt DNA, nuclear DNA [4] and epigenetic elements [5]. C1, which is made up of 44 subunits, 37 of which is encoded by nuclear DNA and the rest by mt DNA [6]. However, most c1 mutations have been associated with mt DNA, rather than nuclear DNA, The reactive oxygen species (ROS) form c1 mutations could cause further mt DNA mutations [7]. In these processes, many factors that affect tissue aging and dysfunction are influenced.
ROS are formed in the respiratory chain that normally occurs in the inner mitochondrial membrane, where five multi-molecular electrochemical generators create a gradient that converts adenosine diphosphate (ADP) to adenosine triphosphate (ATP) due to mitochondrial oxidative phosphorylation (OXPHOS) [8]. C1 and C3 are the main producers of ROS [8]. Mitochondria are the main producers of ROS. One difference between nuclear and mt DNA, is that mitochondria lack histones, making mt DNA very susceptible to damage from ultraviolet (UV) rays and ROS [9]. These oxidants might cause a decrease in the electron transport chain (ETC) enzyme activity, inefficiency in ATP production, impaired cellular respiration, nuclear/mt DNA mutations and tissue damage [10].
The other component of the mitochondria is the sirtuin family, specifically sirtuin 3 (SIRT3) [11]. SIRT3 activates acetyl-coA synthetase, which forms acetyl-coA-Acetyl-coA is essential for protien,sugar, and lipid metabolism. SIRT3 resides in the matrix of the mitochondria and mice lacking SIRT3 develop cancer [12]. SIRT3 a protien anti-oxidant, has been shown to protect tissue from ROS [13].
Finally, mitophagy, or the self-destruction of dysfunctional mitochondria plays a large role in health. If the dysfunctional mitochondria are not eliminated, then excessive ROS is produced, causing tissue damage [14].
A balance between the ROS, protective mechanisms from ROS in times of infection, and ultimately mitophagy equal the overall cellular and tissue health ( Figure 2). One mechanism of mitochondrial dysfunction is a disruption of mitophagy. Mitophagy is regulated by mechanistic target of rapamycin complex 1 (mTORC1) [15], which has numerous blockers, like rapamycin. Rapamycin can induce mitophagy [16]. In Parkinson's disease, two proteins are known to promote mitophagy in dysfunctional mitochondria: PARKIN and PINK1 [17]. Mutations in PARKIN and PINK1 can lead to an accumulation of dysfunctional mitochondria, an increase in ROS, and Parkinson's symptoms.

Epigenetics in mitochondria
The epigenetic phenomenon is a nuclear phenomenon, encompassing methylation, micro-RNA (non-coding), sRNAs (noncoding), histone modifications, etc. However, there is mounting evidence of genomic epigenetics cross-talk with mtDNA ( Figure  1). Environmental factors, including certain drugs, can influence mtDNA and nuclear DNA in an epigenetic pattern. Due to the above described vulnerability of mtDNA, it is logical that mtDNA is accessible for cross-talk. Reversely, mitochondrial depletion has been associated with altered DNA methylation profiles of certain human nuclear genes. Mitochondrial depletion could down-regulate nuclear DNA repair resulting in DNA damage that is converted into mutations by error-prone repair polymerases.
aminoglycosides, and β-lactams produced toxic levels of ROS that showed damage to DNA, proteins and cellular membrane [18]. Furthermore, the tetracycline family of antibiotics has been shown to cause mitochondrial dysfunction [19]. In animal models, tetracyclines cause mitochondrial proteotoxic stress, causing changes in nuclear gene expression and altered mitochondrial function [19]. The fact that depletion can be induced and then reversed highlights the relevance of epigenetics in mitochondrial health [9].

The role of ultraviolet (UV) light in mitochondrial dysfunction
The number one environmental cause of oculocutaneous aging is UV irradiation from the sun. Mitochondria are especially susceptible to this damage [20]. With aging, the enzymatic activity of mitochondria decreases and there is an increase in mtDNA mutations [21]. mtDNA have a 50-fold greater mutation rate than nuclear DNA [22]. In humans, it is thought that large-scale deletion of mtDNA, and not point mutations, are involved in UV-induced photoaging [23]. This large-scale mtDNA deletion, or as it has become to be known as the "common deletion" does not correlate with chronological age and seems to be UV specific [24]. When human mtDNA mutations are followed over years, the mutations can increase by 32 folds, even without further UV irradiation. Heat also causes mtDNA damage. Infrared (IR) irradiation causes point mutations in mtDNA in a different deleterious mechanism than UVA or UVB [25]. This is thought to cause a "defective power house" model of premature aging, where there is altered collagen production by fibroblasts, neovascularization and inflammation, all hallmarks of ocular surface aging and dry eye [10].

The role of mitochondrial dysfunction in uncontrolled growth of tissue
Mitochondria have a role in uncontrolled growth of tissue.
For example, tumors are in desperate need of energy and macromolecules, so it is not surprising that mitochondria are important to tumor growth. Mitochondrial dysfunction is intimately involved in skin cancers. Incidence of mtDNA mutations in melanoma and head and neck squamous cell carcinoma has been shown to affect every mitochondrial gene [26]. Keratinocyte differentiation and propagation by Notch and β-catenin signals are regulated by ROS. Their signaling pathway is also important in skin appendageal development and tumorigenesis [27]. For example, mitochondria are rich in basal cell carcinomas [28], However, ubiquinone, which is important in tagging cells for mitophagy, is consistently downregulated in basal cell carcinomas [29]. Oncocytomas, which are growths associated with Birt-Hogg-Dube syndrome and Cowden Syndrome, are associated with mutations in c1, causing excessive ROS production [30].
The ROS activates a complex structure called the inflammasome. The Inflammasome regulates caspase-1-dependent secretion of IL-1; this process promotes skin cancer formation [31]. The most common BRAF mutation in melanoma induces dysfunctional OXPHOS, and primary and metastatic melanomas have higher expression of OXPHOS than nevi [32]. Mitophagy may have a role in melanoma progression [33]. Therefore, dysfunction in mitochondria is associated with some skin cancers and syndromes associated with internal malignancies.

The role of mitochondria on the ocular surface
ROS is elevated in dry eye syndromes [34]. Numerous factors can cause an elevation of ROS, but mitochondria are the number one producers of ROS in humans, especially as the mitochondria becomes less functional [35]. For example, dry eye has a reduction of lacritin, a tear glycoprotein needed to induce autophagy in situations of inflammation [36]. The reduction of lacritin in dry eye creates a vicious cycle of inflammatory cells creating greater ocular surface damage (Table 1) [37]. Corneal disease in association with mitochondrial dysfunction have included astigmatism, corneal clouding in Fanconi syndrome [38], corneal dystrophy and corneal endothelial dysfunction in Pearson syndrome [39].
In cultured corneal epithelium, increase in ROS results in mitochondrial DNA damage [40]. Oxidative stress can have a role in pterygium formation [41], Fuchs' endothelial dystrophy [42] and corneal dystrophy [43]. Fuchs' endothelial dystrophy, that has a mutation in COL8A2, has been associated with mtDNA damage [44]. Thrombospondin 1 (THBS1) gene mutation can also cause mitochondrial dysfunction [45]. and dry eye [46]. In a Tet-mev-1 mouse model of dry eye, mev-1 is conditionally expressed, This causes mitochondrial dysfunction in the lacrimal glands reduced tear production and increased ROS production. Mev-1 produces Cyt-1, which is part of C2 of mitochondria l OXPHOS. This has been shown to increase ROS, ocular surface damage, and decreased tear function (Figure 3) [49].
In another animal model, SIRT1, which is a component of mitochondria, was downregulated, and this resulted in tear film abnormalities, increased corneal fluorescein staining and ultimately dry eye disease [50].
In another animal model, rats underwent low humidity to induce dry eye, and were followed by stress markers Rab3d, Rab27b, Rab3d and syntaxin [51]. However, if the rats were placed on a calorie-restricted diet, which causes mitophagy [52], they had improved lacrimal function and healthier tear composition using the phenol red thread test post carbachol stimulation [53].
In humans, Sjøgren's Syndrome (SS) has been associated with mitochondrial dysfunction [54]. SS causes a vicious cycle of reduced lacrimal function, and the reduced tear production induces more ROS formation. This cycle causes a pathologic level in keratoepitheliopathy scores, goblet cell density and Schirmer tear value, all translating into dry eye [55].
A clinical study of 20 patients revealed that keratoconus was due to ROS from mitochondrial dysfunction in C1 [56]. When blink-suppressed dry eye is induced, a direct relationship to ROS is identified [57], causing surface inflammation [58], and decreased antioxidant enzymes levels [59]. Conjunctivitis complete ptosis, spontaneous corneal ulceration and keratitis have been reported in Kearns-Sayre syndrome [60], which is known to have mitochondrial DNA deletions [61]. Those having mtDNA deletion at bp 3895 had the greatest deletion load in the cornea [62]. Furthermore, endothelial or Descemet membrane abnormalities have caused

Am J Biomed Sci & Res
Copy@ Omeed M Memar. 177 corneal clouding in Kearns-Sayre syndrome [63]. Leigh syndrome, that can cause non-specific corneal changes, was documented to have abnormal appearing mitochondria and a mutation in mtDNA bp 8993 [64]. Other syndromes with mitochondrial dysfunction have shown ocular pathology. Mitochondrial neurogastrointestinal encephalopathy syndrome have shown nonspecific corneal changes [65]. Pontocerebellar hypoplasia can have mitochondrial aminoacyl-tRNA synthetases mutations [66].
Patients with Gaucher disease, with mutation of glucocerebrosidase, can present with corneal opacification [67] and mitochondrial dysfunction [68]. Optineurin is yet another important element for mitophagy. Abnormal optineurin expression has been documented in keratoconus formation [69]. TGF-β1 has been shown to induce mitochondrial dysfunction and pro-fibrotic conditions [70]. In Marfan syndrome, a defective fibrilin causes increased levels of TGF-β1, and symptoms, such as flattened and thinned cornea can be seen.

The role of anti-oxidants in ocular surface health
SkQ1 is an anti-oxidant that is mitochondria specific. When SkQ1, is applied to the eyes in a rabbit model, ROS production is prevented, TNF-α and IL-6 is downregulated and IL-10 is upregulated. Corneal apoptosis is hence reduced. Furthermore [71]. resveratrol, which can induce mitophagy [72], has been shown to protect corneal epithelium from cytotoxic agents [73]. Polyunsaturated fatty acids in an animal model altered corneal neovascularization [74], but other studies have failed to reproduce these findings [75]. However, rapamycin, which regulates mitophagy, has shown to extend the survival of corneal cells in culture [76], and reduced scarring after keratectomy [77]. In vitro, rapamycin increased the colony forming efficiency of corneal cells [78] and reduced corneal cell senescence [79]. Ceramides are essential to corneal health [80], and their presence induce mitophagy and even tumor suppression [81].

Conclusion
In review of the published literature, mitochondria are intimately associated with oculocutaneous health and disease. We have reviewed the literature on the different diseases associated with mitochondrial dysfunction. We have also presented the current concepts of how mitochondria become dysfunctional. We already have a number of medications that act upon the mitochondria and alter mitochondrial function [82]. Many herbals touted as promoters of longevity and health, like resveratrol, are known to influence mitochondrial health. Now that the mechanisms are becoming clearer, we can use existing and newly designed drugs to improve mitochondrial health and with it, ocular surface and cutaneous health.