Fuel and Run: Metabolism in Neutrophil Migration

Neutrophils are an essential component of the host innate immune system. Migration of neutrophils into inflicted sites composes the primary defense line against pathogens. Recent studies indicate that neutrophils utilize multiple metabolic pathways, instead of only glycolysis. Importantly, neutrophils may preferentially adopt a certain metabolic pathway to adapt to the altered cellular environment or upon various stimuli. In addition, recent studies have shown mitochondrial and TCA cycle are indispensable for neutrophil migration, and here we briefly discuss the regulation of neutrophil migration through cellular metabolism.


Introduction
Neutrophils, the most abundant circulating leukocytes in human, are the first line of host innate cellular defense against invading microorganisms [1]. Neutrophil migration into the inflicted site is critical for pathogen clearance and tissue repair, as aberrant neutrophil migration into tissues causes recurrent infections [2,3] and tissue damage seen in pathological conditions like ischemiareperfusion injury and arthritis [4]. The recruitment of neutrophils into inflicted tissue is a multiple-step cascade, including neutrophil tethering, rolling, adhesion, crawling, and extravasation. In inflicted tissue neutrophils execute microorganisms through phagocytosis, oxidative burst, cytokines secretion, and neutrophil extracellular traps (NETs). Regulation of neutrophil migration has been excellently reviewed [1,5,6] and here we briefly summarize recent advancements on cellular metabolic insights in neutrophil migration.

Metabolic pathways in neutrophils
Neutrophil metabolism has not received much attention for long time as neutrophils are terminally differentiated short-lived cells. Previously, glycolysis was considered as the only metabolic pathway in neutrophils [7]. During glycolysis, low levels of cholesterol levels are reduced [11][12][13][14]. Meanwhile, neutrophils are capable of altering their metabolism to adapt to the changed environment. For instance, resting and activated neutrophils express glucose transporters GLUT1, GLUT3 and GLUT4 differently [15]. Under glucose-limiting conditions, neutrophils utilize fatty acids and glutamine to generate acetyl CoA through mitochondrial

Abstract
Neutrophils are an essential component of the host innate immune system. Migration of neutrophils into inflicted sites composes the primary defense line against pathogens. Recent studies indicate that neutrophils utilize multiple metabolic pathways, instead of only glycolysis. Importantly, neutrophils may preferentially adopt a certain metabolic pathway to adapt to the altered cellular environment or upon various stimuli. In addition, recent studies have shown mitochondrial and TCA cycle are indispensable for neutrophil migration, and here we briefly discuss the regulation of neutrophil migration through cellular metabolism. fatty acid β oxidation and glutamine oxidation, respectively [16,17].
Thus, metabolic plasticity helps neutrophils survive and function under diverse stresses.

Metabolic regulation of neutrophil transmigration
Neutrophil migration is important for the removal of invaded pathogens and healing of injured tissues during immune responses. During migration, neutrophils consume huge amount of energy, particularly for the cytoskeleton reorganization events like neutrophil adhesion, polarization, and crawling [1,2,5,6].
Coupling with the actin cytoskeleton is critical for integrindependent mechanotransduction, which is required for neutrophil firm adhesion [18,19]. Neutrophils are polarized when cells are crawling on the surface of blood endothelial cells by forming lamellipodia in the leading edge and uropod at the tail, which are enriched with actin and myosin, respectively. It is believed that polarization is essential for directional migration of neutrophils [20]. The cytoskeleton reorganization is regulated by small GTPases [21,22] which commonly requires energy to function properly [23]. It has been reported that mitochondrial and TCA enzymes regulate neutrophil migration [24,25]. Treating neutrophils with an uncoupler of mitochondrial oxidative phosphorylation FCCP [26] or mutating iso-citrate dehydrogenase (IDH), an enzyme in the TCA cycle metabolism [27] reduces ATP production and leads to impairment of neutrophil migration. Studies have revealed that ATP released at the leading edge of crawling neutrophils amplifies chemokine signaling and controls gradient sensing and migration through P2Y2 and A3 receptors in an autocrine feedback manner [28]. Further studies have shown that binding of ATP to P2Y2 also promotes mTOR signaling to enhance mitochondrial activity at the leading edge of neutrophils [24]. In summary, mitochondria and the TCA cycle regulate neutrophil migration through producing ATP that is required to reorganize the cytoskeleton during neutrophil migration.

Future Prospects
There is growing amount of evidence indicating that neutrophils utilize multiple metabolic pathways, and cellular metabolism is critical for neutrophil migration. However, many questions still remain to be addressed in this cutting-edge field. For example,

My research project was sponsored by American Heart
Association (18CDA34120009).