Covid-19 (SARS-CoV-2) VS Sars-CoV; Summary of all things that healthcare providers should know

The corona viruses are members of a family of enveloped viruses that replicate in the cytoplasm of animal host cells. They are distinguished by the presence of a single-stranded plus-sense RNA genome about 30 kb in length that has a 5′ cap structure and 3′ polyadenylation tract. Upon infection of an appropriate host cell, the 5′-most open reading frame (ORF) of the viral genome is translated into a large polyprotein that is cleaved by viral-encoded proteases to release several nonstructural proteins, including an RNA-dependent RNA polymerase (Rep) and an adenosine triphosphatase (ATPase) helicase (Hel). These proteins, in turn, are responsible for replicating the viral genome as well as generating nested transcripts that are used in the synthesis of the viral proteins. The mechanism by which these subgenomic mRNAs are made is not fully understood. The SARS-CoV genomes are about 30 kb in length. Its large RNA genome encodes four structural proteins, sixteen non-structural proteins and eight accessory proteins. SARS-CoV is a plus-strand RNA virus featuring a large single-stranded RNA genome of approximately 29 700 nucleotides. The genome is predicted to consist of atleast fourteen functional ORFs that encode three classes of proteins: two large polyproteins, pp1a and pp1ab, which are cleaved into sixteen non-structural proteins (nsps) required for viral RNA synthesis (and probablyother functions); four structural proteins (the S, E, M and N proteins), essential for viral assembly; and eight accessory proteins, which are thought unimportant in tissue culture but may provide a selective advantage in the infected host.


Introduction
In late 2019, several patients with severe pneumonia were brought into hospitals across the City of Wuhan. The virus causing the pneumonia was sequenced and it was found out that it is a strain of beta-coronavirus and most similarly related to SARS-like BAT coronaviruses bat-SL-CoVZC45 and bat-SL-CoVZXC21 with 88% similarity, 79.5% homology with SARS, and 50% with MERS [1,2]. As a virus transmitted from person to person, as time goes by it inevitably mutates and potentially virulent strains might emerge with high mortality rate [3].
The severe acute respiratory syndrome (SARS) is a novel infectious disease that first occurred in November 2002 in China. SARS is caused by a newly emerged virus belonging to the coronaviridae family, provisionally termed SARS-coronavirus (SARS-CoV) [4]. Available sequence data indicate that SARS-CoV is clearly different from all previously known coronaviruses [5]. Most cases were the result of direct transmission via respiratory droplets during close personal contact, and adequate respiratory protective measures were shown to be effective [6]. In mainland China, around 50% of probable SARS patients did not have an apparent history of close personal contact with another case [7]. At least a proportion of these cases might have arisen from modes of transmission other than droplets.

Methods and Data Gathering Epidemiology
As of 24 January 2020, 1287 cases of novel coronavirus (2019-nCoV) infections were reported in mainland China, causing 41 deaths. While infections in the first case cluster were initially thought to be mostly due to zoonotic (animal-to-human) transmission-possibly due to wild animals sold at a local seafood wholesale market [8,9] -the growth of case incidence in Wuhan after closure of the market and exportation of cases across China and internationally shows compelling evidence of increasing human-to-human secondary transmission, fueled by human migration. Cases have now been detected in many other parts of the world [10], including other Asian countries, the United States, and France.

Abstract
The corona viruses are members of a family of enveloped viruses that replicate in the cytoplasm of animal host cells. They are distinguished by the presence of a single-stranded plus-sense RNA genome about 30 kb in length that has a 5′ cap structure and 3′ polyadenylation tract. Upon infection of an appropriate host cell, the 5′-most open reading frame (ORF) of the viral genome is translated into a large polyprotein that is cleaved by viralencoded proteases to release several nonstructural proteins, including an RNA-dependent RNA polymerase (Rep) and an adenosine triphosphatase (ATPase) helicase (Hel). These proteins, in turn, are responsible for replicating the viral genome as well as generating nested transcripts that are used in the synthesis of the viral proteins. The mechanism by which these subgenomic mRNAs are made is not fully understood. The SARS-CoV genomes are about 30 kb in length. Its large RNA genome encodes four structural proteins, sixteen non-structural proteins and eight accessory proteins. SARS-CoV is a plus-strand RNA virus featuring a large single-stranded RNA genome of approximately 29 700 nucleotides. The genome is predicted to consist of atleast fourteen functional ORFs that encode three classes of proteins: two large polyproteins, pp1a and pp1ab, which are cleaved into sixteen non-structural proteins (nsps) required for viral RNA synthesis (and probablyother functions); four structural proteins (the S, E, M and N proteins), essential for viral assembly; and eight accessory proteins, which are thought unimportant in tissue culture but may provide a selective advantage in the infected host.

Copy@ Seyed Ali Hossein Zahraei
The incubation period is defined as the time from infection to illness onset. Knowledge of the incubation period of a directly transmitted infectious disease is critical to determine the time period required for movement restriction of healthy individuals (i.e. quarantine period) [11,12]. A published clinical study has already shown that the average time delay from illness onset to admission is approximately 7 days [13].
Our results show that 95% of incubation periods fall within the range of 2 to 9 days, and the median incubation period was 4-5 days when the Weibull distribution was used as the best-fit model [14]. The median time from illness onset to hospitalization was approximately 3 days. The median time from illness onset to death was 13.8 days, the delay of which is key to appropriate estimation of the case fatality risk for 2019-nCoV [15].
Our estimated median incubation period of 2019-nCoV is comparable to known median values of the incubation period for severe acute respiratory syndrome (SARS)-estimated at 4.0-6.4 days [16][17][18]. In addition to empirically showing the comparability to SARS, the present study has also shown that the 95 th percentile of the incubation period is around 7-8 days, indicating that a nineday quarantine period could mostly ensure the absence of disease among exposed healthy individuals. The time from illness onset to death is also comparable to SARS [14], and the 13.8-day median delay that we calculated indicates that the crude estimation of the ratio of the cumulative number of deaths to that of cases tends to result in underestimation of the case fatality risk, especially during the early stage of the epidemic.

Sars-CoV
Epidemiology: Since November, 2002, an infectious agent has caused outbreaks of an atypical pneumonia in Guangdong Province, southern China [19]. The illness usually began with high fever and mild respiratory symptoms, but rapidly progressed to pneumonia within a few days [20] At the end of February, 2003, the disease had spread to neighbouring regions and countries [21,22]. The disease was severe, transmissible from person to person, and seemed to cause clusters of disease in health-care workers. It was named

Genetics
The corona viruses are members of a family of enveloped viruses that replicate in the cytoplasm of animal host cells [29].

Copy@ Seyed Ali Hossein Zahraei
The viral membrane proteins, including the major proteins S The virus then migrates through the Golgi complex and eventually exits the cell, likely by exocytosis [29]. The site of viral attachment to the host cell resides within the S protein.
The SARS-CoV genomes are about 30 kb in length [36]. Its large RNA genome encodes four structural proteins, sixteen nonstructural proteins and eight accessory proteins [37]. SARS-CoV is a plus-strand RNA virus featuring a large single-stranded RNA genome of approximately 29, 700 nucleotides [38,39] The genome is predicted to consist of atleast fourteen functional ORFs that encode three classes of proteins: two large poly-proteins, pp1a and pp1ab, which are cleaved into sixteen non-structural proteins(nsps) required for viral RNA synthesis (and probably other functions); four structural proteins (the S, E, M and N proteins), essential for viral assembly; and eight accessory proteins, which are thought unimportant in tissue culture but may provide a selective advantage in theinfected host [40]. The SARS corona virus includes four structural proteins that are required to drive cytoplasmic viral assembly: the spike (S) protein, the membrane (M) protein, the nucleo-capsid (N) protein and the envelope (E) protein [38,39].

Prevention
The wearing of face masks is the single most important precaution against SARS in hospital settings [42] A case-control

Am J Biomed Sci & Res
Copy@ Seyed Ali Hossein Zahraei study among 241 non-infected and 13 infected staff members with documented exposure to 11 index SARS patients suggested that wearing a face mask is the most important infection control tool, followed by appropriate hand hygiene, [43] suggesting that droplets and hands play a major role in transmission of SARS-CoV. Hands may be contaminated by patient excretions or contact with contaminated surfaces. From the inanimate environment, nosocomial pathogens can be transmitted to hands quite easily [44]. The World Health Organization (WHO) has suggested that standard disinfectants should be effective against SARS-CoV [45].

Discussion and Conclusion Incubation
Reports from member laboratories of the WHO SARS network indicated that the virus is stable in faeces and urine at RT for at least 1-2 days and even more stable (up to 4 days) in stool from patients with diarrhoea (which has a higher pH than normal stool) [47,48]; Thermal inactivation of SARS-CoV at 56° and 60°C is highly effective; however, in the presence of protein (20%), infectivity was only reduced by less than 2log10 at 56°C after 30 min (and also after 60 min; data not shown). This has implications for the handling of clinical specimens from SARS patients; for example, pre-treatment of sera at 56°C for 30 min might not be sufficient for full inactivation. On the other hand, as SARS-CoVviraemia does not seem to reach high titres [49], a moderate reduction of residual infectivity might be sufficient to enable the safe performance of serological laboratory assays.
SARS-CoV has been described to persist on surfaces for up to 96 h [50]. In another study, dried SARS-CoV retained its infectivity for as long as six days, indicating a relatively strong survival ability.
Only after nine days in a dried state did SARS-CoV completely lose its infectivity [51]. At admission, all patients had fever, sometimes accompanied by myalgia and headache (Table 1). Other symptoms, including dry cough, developed 2-4 days after onset of fever. Shortness of breath (when present) generally manifested in week 2 of illness [52].

Treatment
Interferons, a family of cytokines important in the cellular immune response, have been shown to be partly effective against animal and human corona viruses [54][55][56]. Interferon b was found to be more potent than interferon α or γ, and it remained effective after viral infection, although the potential difference between interferon a and b has been debated [57]. The use of interferons in the treatment of SARS has been limited to interferon a in combination with steroids, immunoglobulins, or thymic peptides, and its efficacy cannot be ascertained [58,59].
A lopinavir-ritonavir co-formulation (Kaletra) is a protease inhibitor preparation used to treat human immunodeficiency virus (HIV) infection. It was used in combination with ribavirin in some Hong Kong hospitals in the hope that it would inhibit coronaviral proteases, thus blocking the processing of the viral replicase polyprotein and preventing the replication of viral RNA. Preliminary results suggest that the use of lopinavir-ritonavir simultaneously with ribavirin and corticosteroids might reduce intubation and mortality rates, especially when administered early [60].
Glycyrrhizin, which is used in the treatment of chronic hepatitis and is relatively nontoxic, has been tested in vitro and found to be an active agent against SARS-CoV [61]. It inhibited viral adsorption and penetration, and was most effective when administered both during and after the viral adsorption period.
Gamma immunoglobulins were used in some hospitals in China and Hong Kong [58]. However, because other therapies such as corticosteroids were often used concomitantly, their effectiveness against SARS remains uncertain.
Noninvasive positive pressure ventilation (NIPPV) was commonly employed in many Chinese hospitals, [58] and was found to avert the need for intubation and invasive ventilation in up