Disease–Free Seed Ginger Production Technology (G3, G4 And G5) in the Field

5-7 Abstract Increasing threats of ginger from bacterial wilt: Ralstonia solanacearum , is impacted to ginger production and market of farmer in Thailand. The objective of this study was to find the integrated ginger production system that are produced high yield stability and able to replanted in the same area for farmer’s well-being sustainable development. This study evaluated the integrated ginger production technology consist of cultivate management, bioproduct from Bacillus subtilis BS-DOA 24 strain for controlling bacterial wilt of ginger and disease-free rhizomes seeds from tissue culture of ginger three generations (G3, G4 and G5) at Phetchabun Highland Agricultural Research Center during October 2016- September 2019. The rhizomes seed production after planting 11 months was free from bacterial wilt disease. The sprouting ginger seed germination percentage after 2 months of G3, G4 and G5 were 92, 97 and 99%, respectively. Total yield of DOA’s technology in G3, G4 and G5 showed 3,248, 10,133 and 10,568 kg/rai (20.3, 63.33, and 66.05 tons/hectare), respectively that the yield of DOA’s G5 was significantly higher than farmer method (6,725 kg/rai or 42.03 tons/hectare). in DOA technology (433 g/rhizome, respectively) was lower than another treatment however DOA’s G5 (1,409 g/rhizome) was significantly heavier than farmer’s technology (910 g/rhizome). The production costs of rhizomes ginger in G3 (28,817 baht/rai or 6,003.54 USD/hectare) were higher than another treatment due to high value of seeds prices in this generation. Net income of DOA’s G5 showed the highest of value as 211,360 baht/rai or 44,033 USD/hectare therefore the profit (186,060 baht/rai or 38,762.5 USD/hectare) also was higher than farmer’s technology. Moreover, 19 farmers were established field demonstration by using DOA’s techniques and these knowledges were transferred to 137 farmers for apply in their farming. Diseases-free rhizome seeds (9,040 kg) were planted in 20.1 rais (3.216 hectare) of farmer fields at Khao Kho, Phetchabun and Kantharawichai, Maha Sarakham. In summary, the disease-free ginger production was shown to increase productivity, decrease unit cost and increase net income and net profit, and BS-DOA 24 strain bioproduct was controlled bacterial wilt in all generations after replanted in the same area.

the rhizome gently [5]. According to the problem, the farmers planted to new lands for ginger production due to avoid bacterial wilt. They used seed rhizome reserved from own production then often infected with this disease [6]. Farmer replanting ginger three generations in the same area showed that, the percent yield in the first year as 80%, reduced 50% in the second year and remained 30% in the third year [7]. Moreover, the disease-free seed rhizome from tissue culture quite small size then the affect to high seed prices and high unit cost [8].
Integrated ginger production system, seed rhizome from tissue culture technique and bioproduct from Bacillus subtilis BS-DOA 24 strain that able prevented bacterial wilt (approximately 60%) in the field [9]. In addition, the management of soil preparation with soil fumigation was mixed with urea fertilizer (0-0-46) and lime rate of 80:800 kg/rai or 0.5:5.0 tons/hectare (1:10 ratio) to reduce the population of bacteria R. solanacearum before planting ginger and during ginger plant [10]. Furthermore, B. subtilis also increased the yield of ginger [11] and potato [12]. The objective of this study was to finding integrated ginger production system that are produced high yield stability and able to replanted in the same area for farmer's well-being sustainable development, environmental conservation and natural resource recovery.

Plant material
The integrated ginger production of DOA's technology, diseasefree rhizomes seeds from tissue culture of three generations (G3, G4 and G5) combined with soil preparation (urea: lime in a ratio of 1:10) and bioproduct from Bacillus subtilis BS-DOA 24 strain for controlling bacterial wilt of ginger from the Department of Agriculture (DOA), Thailand were compared with farmer's technology ( Table 1)

DOA Ginger Production Technology
In the first year, the G3 disease-free seed rhizome of ginger production was produced from G2 seed rhizome in Chiang Rai Horticulture Research Center, Mueang, Chiang Rai during 2016-2017. After removed weeds, the management of soil preparation in one rai (0.16 hectare) with soil fumigation was mixed with urea fertilizer (0-0-46) and lime rate of 80:800 kg/rai or 0.5:5.0 tons/ hectare (1:10 ratio). The land was plowed two times and solarized of beds in four weeks for decrease the pathogens and contamination.
Beds of about 70 cm width, 25 cm height and convenient length were prepared with an interspace of 70 cm in between beds.
Chicken manure were applied in furrow before planting with 250 kg/rai (1.56 tons/hectare). The seed rhizomes 7,600 plants/rais or 47,500 plants/hectare were cut into small pieces of 5-7.5 cm length each having three or four good sprouts.
These seeds were dipped with mancozeb fungicide (50 mL/20 L of water) and cypermethrin pesticide (50 mL/20 L of water) for 30 minutes, shade dried for 30-60 minutes after that treated with B. subtilis 'BS-DOA 24' (40 mL/ 20 L of water) for 30 mins, shade dried. The seed rhizome bits were planted at a spacing of 30 × 70 cm and placed in shallow pits and covered with a thin layer of soil (2-5 cm). Mulching the bed with rice straw or grass straw were covered the row for protect the moisture, sun burning, and weed competition then an enclosed 1 m high of the net fence to protect from human and animal contamination. NPK fertilizers (15-15-15) was applied in the rate of 50 kg/rai (0.31ton/hectare) 2 months after planting and 50 kg/rai (0.31 ton/hectare) of NPK (13-13-21) was applied at 4 months after planting in a furrow after removed weed, and made a horn. Spraying BS-DOA 24 bioproduct (40 mL/ 20 L of water) every month continue to four months. However, the infection of bacterial wilt in ginger plantlet was close from the field and combined lime and urea (46-0-0) with 10:1 ratio at 500 g/ plant.
After that, covered hold with soil and applied BS-DOA suspension (40 mL/20 L of water) was applied at 30-50 mL/plant in the evening until the disease symptom appearing. The cultural and management practices i.e. irrigation and spraying for insect pest and disease control were carried out uniformly for all treatments.
The ginger plants were harvested at 10-11 months after planting or when the plant's foliage or stem had died back. After collection, the seed rhizomes were determined. The G4 seed rhizomes were stored in the shade after 2 months for produced G5 seed rhizome production and testing the integrated technology of DOA's ginger production compared with farmer's technology in PBHARC during 2018-2019 (Table 1).

Farmer's ginger production technology
In the third year, the G4 seed rhizome of farmer were compared with the integrated technology of DOA's ginger production in PBHARC during 2018-2019 (Table 1) The seed rhizome bits were planted at a spacing of 30 × 70 cm and placed in shallow pits and covered with a thin layer of soil (2-5 cm). Mulching the bed with rice straw or grass straw were covered the row for protect the moisture, sun burning, and weed competition then an enclosed 1 m high of the net fence to protect from human and animal contamination. NPK fertilizers (15-15-15) was applied in the rate of 50 kg/rai (0.31 ton/hectare) 2 months after planting and 50 kg/rai (0.31 ton/hectare) of NPK (13-13-21) was applied at 4 months after planting in a furrow after removed weed, and made a horn. The cultural and management practices i.e. irrigation and spraying for insect pest and disease control were carried out uniformly for all treatments. The ginger plants were harvested at 10-11 months after planting or when the plant's foliage or stem had died back. After collection, the seed rhizomes were determined.

The Extension and Transfer of Technology
Knowledge management in the integrated ginger seed rhizome production of DOA's technology was prepared for transfer to farmers. The seed rhizome field demonstration was established in

Statistical analysis
The data included the percentage of sprout germination, number of branches per rhizome, weight per rhizome, production per rai and cost were recoded. The experiment was laid out using a randomized completely block design (RCBD) and analyzed by using the independent t-test (p≤0.05) with two treatments and four replications. Statistical analyses were carried out using the IRRISTAT program.

Testing Integrated Technology of Ginger Production The Yield Components and Total Yield of Ginger
Sprout germination rate of ginger rhizome seed in raised beds after planting 2 months increased in each generation ( Table   2). DOA's rhizome seed in G3 generation showed 92% sprout germination, 97% in G4 and 99% in G5. In addition, G5 rhizome seed of DOA's technology was lower sprout germination than seed of farmer's technology (98%). Sprouts start to germinate because both respiration and moisture loss increase rapidly [13]. Ginger is a quantitative short-day plant and that long days tend to enhance vegetative growth while rhizome swelling is promoted by short days [14]. The optimum soil temperature for germination is between

DOA's Ginger Production Technology
Farmer's Ginger Production Technology 1 Using the disease-free rhizomes seeds from tissue culture of G3, G4 and G5. Using the ginger rhizomes seed that selected from the year before planting.
2 Soil preparation: replanting in the same area with disinfects of pathogens (urea fertilizer and lime rate of 1:10 ratio). Planting in the new area due to avoid pathogens.

Copy@ Jitarpa Jijuban
The number of branches per rhizome decreased in each generation due to initial generation (G3) of seed size represented small size and became large in G5 (Table 2). Then, branches number in G5 of DOA's technology (7 branches/rhizome) was less than G5 of farmer's technology (9 branches/rhizome) but not significantly different from the farmer treatments. However, the rhizome size of DOA's technology was larger than rhizome of farmer's technology.
These results are similar with the study of [16] revealed that ginger plantlets from meristem micropropagation produced more tillering and rhizome branching when compared with conventional method (old rhizomes). The weight of rhizome in G3 (433 g/rhizome), G4 (1,351 g/rhizome) and G5 (1,409 g/rhizome) increased in each generation ( These results are similar to those of [11] reported that BS-DOA 24 improved the ginger production at 4,288.03 kg/rai which more than control [12] indicated that the application of a microbial product of B. subtilis strain increased tuber yield by fostering the recruitment of beneficial bacteria in the potato rhizosphere [12].
In addition, B. subtilis had properties to be plant growth promoting microorganism and mechanism to suppress disease caused by pathogen which increase crop productivity [18].

The Production Costs of Ginger
The important production cost of ginger production was the rhizome seed in each generation ( Table 3)  contributed higher to the total variable cost of production [20].

Conclusion
The integrated ginger production DOA's technology was shown to be an appropriate method for soil preparation (urea: lime in a ratio of 1: 10) combine with disease-free rhizomes seeds from tissue culture of three generations (G3, G4 and G5) and bioproduct from B. subtilis BS-DOA 24 strain for controlling bacterial wilt of ginger.
This technology increased yield and rhizome weight and decreased the total costs of ginger production. The farm income increased then increased quality of a livelihood in the farming households.
Moreover, 19 farmers were established field demonstration by using DOA's techniques and these knowledges were transferred to 137 farmers for apply in their farming.